Key Highlights :
1. The cortex is a thin layer of filamentous actin, myosin motors, and regulatory proteins beneath the plasma membrane.
2. The mechanical properties of the cortex are key to its physiological function.
3. Changes in cortical mechanics can originate from changes in the architecture of the actin network.
4. However, the complete inventory of assembly factors driving formation of the actin cortex and how their activities are spatiotemporally controlled are not well understood.
5. In a study published in the Journal of Cell Biology, Prof. Cai Huaqing's group at the Institute of Biophysics of the Chinese Academy of Sciences uncovered a signaling cascade responsible for building the rear actin cortical subcompartment in rapidly migrating cells.
6. Using Dictyostelium as a model for polarized and rapidly migrating cells, the researchers found that a RhoGEF domain-containing protein named GxcM localizes specifically in the rear of directionally migrating cells.
7. Overexpression of GxcM induces excessive actin polymerization in the rear cortex, and these structures can be marked by the Arp2/3 complex, suggesting that GxcM possesses the ability to promote branched actin assembly.
8. By generating a series of truncations and mutations, they demonstrated that the function of GxcM relies on its C-terminal proline-rich motifs and GEF activity.
9. Co-immunoprecipitation, mass spectrometry , and microscopic imaging experiments showed that GxcM binds to and recruits F-BAR protein, Fbp17, through its C-terminus.
10. In fbp17 knockout cells, GxcM still localizes in the posterior of the cell, but loses the ability to stimulate actin polymerization, resulting in a decrease in cortical actin content, a significant reduction in the cell's ability to resist external mechanical forces, and defects in cell division and migration.
11. Biochemical and in vitro actin polymerization experiments showed that Fbp17 is capable of binding and activating the actin nucleation promoting factor WASP through its SH3 domain, which in turn activates Arp2/3 and mediates branched actin polymerization.
12. Additionally, the researchers found that GxcM regulates actin assembly by stimulating the activity of the small GTPase, RacC.
13. Yeast two-hybrid and biochemical experiments showed that active RacC binds to both Fbp17 and WASP.
14. Inducing the expression of constitutively active RacC in cells produces effects similar to GxcM overexpression, in Fbp17- and WASP-dependent manner.
15. Deletion of racC causes phenotypes similar to deletion of fbp17, resulting in defects in
The cortex is defined as a thin layer of filamentous actin, myosin motors, and regulatory proteins beneath the plasma membrane. Assembly and contraction of this actin meshwork generates cortical tension, which enables cells to resist external mechanical stresses, change shape, and exert forces. Consequently, the cortex plays a critical role in a variety of cellular processes, including division, migration and morphogenesis.
The cortex is composed of two main types of cells: epithelial and neuronal. Epithelial cells are responsible for the outermost layer of the cortex, while neuronal cells are located in the center. The cortex is essential for a variety of cellular processes, including division, migration, and morphogenesis.
The cortex is composed of two main types of cells: epithelial and neuronal. Epithelial cells are responsible for the outermost layer of the cortex, while neuronal cells are located in the center. The cortex is essential for a variety of cellular processes, including division, migration, and morphogenesis.
The cortex is composed of two main types of cells: epithelial and neuronal. Epithelial cells are responsible for the outermost layer of the cortex, while neuronal cells are located in the center. The cortex is essential for a variety of cellular processes, including division, migration, and morphogenesis.
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